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Splatter: simulation of single-cell RNA sequencing data.

As single-cell RNA sequencing (scRNA-seq) technologies have rapidly developed, so have analysis methods. Many methods have been tested, developed, and validated using simulated datasets. Unfortunately, current simulations are often poorly documented, their similarity to real data is not demonstrated, or reproducible code is not available. Here, we present the Splatter Bioconductor package for simple, reproducible, and well-documented simulation of scRNA-seq data. Splatter provides an interface to multiple simulation methods including Splat, our own simulation, based on a gamma-Poisson distribution. Splat can simulate single populations of cells, populations with multiple cell types, or differentiation paths.

Splatter: simulation of single-cell RNA sequencing data.

> 商业许可源文 · EUROPE_PMC · [CC-BY](https://creativecommons.org/licenses/by/)

书目信息

  • 引用:Zappia L, Phipson B, Oshlack A. (2017). Splatter: simulation of single-cell RNA sequencing data. Genome biology. PMID 28899397 · PMC5596896 · DOI 10.1186/s13059-017-1305-0
  • 证据类型:PRIMARY_RESEARCH
  • 主题:rna-seq、single-cell
  • 被引次数(采集时):737
  • 原始记录:[Europe PMC](https://europepmc.org/article/MED/28899397)
  • 来源许可:[CC-BY](https://creativecommons.org/licenses/by/)
  • 作者摘要(按来源许可复用)

    As single-cell RNA sequencing (scRNA-seq) technologies have rapidly developed, so have analysis methods. Many methods have been tested, developed, and validated using simulated datasets. Unfortunately, current simulations are often poorly documented, their similarity to real data is not demonstrated, or reproducible code is not available. Here, we present the Splatter Bioconductor package for simple, reproducible, and well-documented simulation of scRNA-seq data. Splatter provides an interface to multiple simulation methods including Splat, our own simulation, based on a gamma-Poisson distribution. Splat can simulate single populations of cells, populations with multiple cell types, or differentiation paths.

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    Splatter: simulation of single-cell RNA sequencing data. · GeniOmics